Analysis of the Enzyme Sucrase for Optimum Temperature, pH, and Concent

Analysis of the Enzyme Sucrase for Optimum Temperature, pH, and Concentration ABSTRACT Enzymes react differently under different conditions and concentrations, being the most productive at the enzymes specific optimum condition and concentration. The enzyme sucrase, extracted from yeast, breaks down the complex sugar sucrose into the simple sugar glucose. Testing for sucrase’s optimum environment, multiple reactions were ran using varying amounts and concentrations of sucrose and sucrase at different pHs and temperatures. The product was then treated with Benedicts solution to visually observe what amount of glucose was present after the reaction was ran; negative results being little to no glucose present and positive results being glucose present. The varying levels of colors created by the Benedicts test were recorded as 1 (negative) blue; 2 green; 3 yellow; 4 (positive) orange; and 5 (positive) red. After running the reactions to determine the optimum temperature and pH, 37 degrees Celsius was determined to be the optimum temperature while pH 2.0 was determi ned to be the optimum pH. This means that under these conditions the enzyme will help produce the most product from the substrate. After finding the optimum conditions the two different concentrations of sucrose were ran for ten minuets taking an initial sample and then subsequent samples every minuet, under those conditions, one concentration at 2.5% sucrose and again at 10% sucrose. The 10% sucrose had the most positive results because there were sufficient active sites to bind with most of the sucrose present producing glucose more efficiently than the 2.5% sucrose. As with the 2.5% sucrose all of the sucrose was bond to the active sites of sucrase and there were ... ...ty Benedict test results. One of these possibilities or some combination can lead to inaccurate optimum temperature and or pH, which in turn would through off the optimum concentration. Over all I feel that the most important part of the optimum environment is the pH because of the competitive inhibition caused by free protons in solution. WORKS CITED Halverson, K. M. and B. A., Barry. 2003. Sucrose and glycerol effects on photosystem 2. Biophysical Journal 85: 1317 Lawrence, S., M. K. Heidemann and D. O. Straney. 2006. Biological Sciences 111L Laboratory Manual. Hayden-McNeil Publishing, Inc., Plymouth Peluffo, D. R.. 2004. Effect of ADP on NA+-NA+ Exchange Reaction Kinetics of Na,K-ATPase. Biophysical Journal 87: 883 Shaevitz, J. W., S. M. Block, and M.J. Schnitzer. 2005. Statistical Kinetics of Macromolecular Dynamics. Biophysical Journal 89: 2277